Coding

Part:BBa_K5519003:Design

Designed by: Latifat Abdulsalam   Group: iGEM24_KFUPM-Saudi-Arabia   (2024-09-16)


BborAMP3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 27


Design Notes

Since this construct is meant to be expressed in Escherichia coli, the sequence of BborAMP1 was codon optimized for expression in E.coli.


Source

BborAMP1 comes from the genome sequence of the thermophilic bacteria Brevibacillus Borstelensis AK1 APBN01000001.1

References